Know more

About cookies

What is a "cookie"?

A "cookie" is a piece of information, usually small and identified by a name, which may be sent to your browser by a website you are visiting. Your web browser will store it for a period of time, and send it back to the web server each time you log on again.

Different types of cookies are placed on the sites:

  • Cookies strictly necessary for the proper functioning of the site
  • Cookies deposited by third party sites to improve the interactivity of the site, to collect statistics

Learn more about cookies and how they work

The different types of cookies used on this site

Cookies strictly necessary for the site to function

These cookies allow the main services of the site to function optimally. You can technically block them using your browser settings but your experience on the site may be degraded.

Furthermore, you have the possibility of opposing the use of audience measurement tracers strictly necessary for the functioning and current administration of the website in the cookie management window accessible via the link located in the footer of the site.

Technical cookies

Name of the cookie

Purpose

Shelf life

CAS and PHP session cookies

Login credentials, session security

Session

Tarteaucitron

Saving your cookie consent choices

12 months

Audience measurement cookies (AT Internet)

Name of the cookie

Purpose

Shelf life

atid

Trace the visitor's route in order to establish visit statistics.

13 months

atuserid

Store the anonymous ID of the visitor who starts the first time he visits the site

13 months

atidvisitor

Identify the numbers (unique identifiers of a site) seen by the visitor and store the visitor's identifiers.

13 months

About the AT Internet audience measurement tool :

AT Internet's audience measurement tool Analytics is deployed on this site in order to obtain information on visitors' navigation and to improve its use.

The French data protection authority (CNIL) has granted an exemption to AT Internet's Web Analytics cookie. This tool is thus exempt from the collection of the Internet user's consent with regard to the deposit of analytics cookies. However, you can refuse the deposit of these cookies via the cookie management panel.

Good to know:

  • The data collected are not cross-checked with other processing operations
  • The deposited cookie is only used to produce anonymous statistics
  • The cookie does not allow the user's navigation on other sites to be tracked.

Third party cookies to improve the interactivity of the site

This site relies on certain services provided by third parties which allow :

  • to offer interactive content;
  • improve usability and facilitate the sharing of content on social networks;
  • view videos and animated presentations directly on our website;
  • protect form entries from robots;
  • monitor the performance of the site.

These third parties will collect and use your browsing data for their own purposes.

How to accept or reject cookies

When you start browsing an eZpublish site, the appearance of the "cookies" banner allows you to accept or refuse all the cookies we use. This banner will be displayed as long as you have not made a choice, even if you are browsing on another page of the site.

You can change your choices at any time by clicking on the "Cookie Management" link.

You can manage these cookies in your browser. Here are the procedures to follow: Firefox; Chrome; Explorer; Safari; Opera

For more information about the cookies we use, you can contact INRAE's Data Protection Officer by email at cil-dpo@inrae.fr or by post at :

INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Menu Logo Principal Ministère de l'agriculture et de l'alimentation Ministère de l'enseignement supérieur, de la recherche et de l'innovation Logo_ANR Centre national de la recherche scientifique Fondation pour la recherche sur la biodiversité Laboratoire LABOGENA : analyses génétiques pour les espèces animales Logo_VetAgro Sup ANTAGENE

Home page

A world first for the protection of wild and domestic donkey breeds

Anes au pré dans l'Aveyron.
Inra researchers, in collaboration with IFCE, CNRS and Université François Rabelais de Tours, have just developed for the first time a technique for in vivo collection of donkey oocytes that can now be used routinely. They also studied the chronology of the in vitro maturation of these oocytes. Published in Theriogenology, their work represents crucial advances in the conservation of the genetic heritage of endangered donkey breeds.

Most wild donkey breeds are on the verge of extinction, such as the Asian wild donkey (Equus hemionus) or the African wild donkey (Equus asinus africanus), mentioned in the Red List of the International Union for Conservation of Nature (IUCN). Many breeds of domestic donkeys in the world are threatened. In France, of the seven breeds of donkeys, five are in the process of extinction with less than 100 females breeding in 2015 (23 Bourbonnaises donkeys, 34 Grand Noir du Berry donkeys, 40 Norman Norman donkeys, 43 Provence donkeys, 88 Cotentin donkeys) and two are in danger of extinction with less than 300 females breeding in 2015 (136 Pyrenees donkeys, 296 Poitou baudets).
The preservation of this genetic heritage is therefore urgent, and involves in particular the cryopreservation of sperm, oocytes and embryos. The cryopreservation of oocytes requires their collection from live females. The production of embryos requires their production in large numbers from a few females, which is not possible in vivo since these animals produce only one embryo per 26-day cycle. This large number of oocytes can be produced in vitro but requires the collection of oocytes from live females. This collection is therefore an essential step for the conservation of the female heritage.
The staff of the UEPAO1 equine platform at INRA succeeded in developing for the first time a technique for collecting oocytes from live donkeys by transvaginal follicular puncture under ultrasound, in agreement with the Val de Loire Animal Experiments Ethics Committee. A total of 92 oocytes were collected during 22 punctures, an average of 4.2 oocytes per donkey. Then, researchers from unit PRC1 adapted an in vitro maturation technique for mare oocytes to donkey oocytes, making it possible to study for the first time the chronology of donkey oocyte maturation. It has been observed that it is possible to obtain 44% of oocytes from mature donkeys after 34 hours of in vitro culture.
This work has therefore made it possible to develop a technique for in vivo collection of donkey oocytes by follicular puncture under ultrasound and to ensure their maturation in vitro: two crucial steps in the conservation of the genetic heritage of species undergoing extinction. Work is currently ongoing on the development of an in vitro fertilization technique to obtain embryos suitable for freezing or transfer to a recipient female for gestation.

See also

The Experimental Unit of Animal Physiology of Orfrasière de l'Inra (UEPAO) and the Joint Research Unit of Physiology of Reproduction and Behaviour (PRC - INRA, CNRS, IFCE, Université François Rabelais de Tours) of the Inra Val de Loire Centre in Nouzilly are involved in this work. This research was funded by the Institut Français du Cheval et de l'Equitation (IFCE) and the CRB-Anim project (Centre de Ressources Biologiques Animales, ANR-11-INBS-0003) as part of the "Investments for the Future" programme.